Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd. Yang Nuryani • Otih Rostiana • Cheppy Syukur. Pengembangan Metode Fusi Protoplas dengan Tingkat Keberhasilan Pembentukan Fusan 25% untuk Memperoleh Tanaman Triploid Jeruk Garut Tanpa Biji. Request PDF on ResearchGate | Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan.

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How to cite item. Language Select Language English Indonesian. OPH 19 and Abi Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Peotoplas patchouli Sidikalang and TT 75and the somatic hybrids.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd

Some fsui of this site may not work without it. Download full text Bahasa Indonesia, 6 pages. Article Tools Print this article. Somatic hybridization by fusion technique is a breakthrough technique that allows the plant generated by an increased level ploidy.

Pusat Penelitian dan Pengembangan Perkebunan Jln. Five random primers, OPD Abstract Tangerine is a citrus seed Indonesia and including a true species of the genus Citrus. Email this article Login required.

Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. View original from ejurnal. Haploid cell population repeatedly sub cultured every 4 weeks in a double layer media: Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian The plant material used for the production of diploid callus is a population of diploid cells induced from embryonic nucellar tangerine.


Metadata Show full item record. Ihe level of resistance of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study was conducted al the laboratory of the Research Institute for food Crop Biotechnology, Bogor in November DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al.

Pre cold treatment for more than 3 days on the response forming callus from pretreatment less than 3 days. Based on the distribution of phenols and lignin content. One of the problems faced in patchouli production is nematode infection Pratylenchus brachyurus. Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs.

Ihe second group 9 clones contained high lignin. JavaScript is disabled for your browser. Meanwhile, from Ihe fusion of Girilaya, Tapak Tuan. This orange tastes sweet but relatively many seeds fruit seed.

In the fusion of Girilaya x Sidikalang. The strategy chosen to assemble the triploid tangerine because the technique of protoplast isolation, fusion and regeneration of tangerine with diploid level has been mastered ploidy. The second group was then classified into 2 minor groups. Index of similarity among genotypes were calculated progoplas using NTsys ver.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd – Neliti

It has been found thai Ihe plain resistance lo nematode is closely proyoplas to phenols and lignin content of the root Therefore. User Username Password Remember me.


Ihe somatic hybrids can be categorized in three group The irst groups 5 clones contained high phenols higher than the average. Abstract Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. Email the author Login required. Sidikalang and TT 75 were tested for their genetic variability and relationship.

Fusi protoplas interspesifik aspergillus Spp

The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram.

S Assessment of genetic variability of patchoulli Protoplaas sp.

Industrial Crops Research Journal. Seedless citrus improvement can be done by manipulating the level ploidy. Plants with triploid ploidy level usually have sterile fruit seedless.

The best enzyme formulations for the isolation of protoplasts from diploid callus and leaves in vitro and protoplast Haploid pollen is macerozim 0. First group consisted protopla somatic hybrids nos.

Phenol and lignin content in the somatic hybrids were dilferenl in Ihe respective pairs. Result showed that Java patchouli Girilaya contained phenols and lignin respectively