INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

Oligosaccharides are compounds with great potential of use in food industry particularly interesting are fructooligosaccharids FOSbecause of their favorable functional properties such as low calorie and noncariogenic sweeteners, improvement of the intestinal microbial flora, relief of constipation, decrease of total cholesterol and lipid in the serum and promotion of animal growth Vandamme and Derycke, A review comparing their physiological effects.

Inulinase can be found in plants and microorganisms. Highly active isolate of Streptomyces grisenus isolated from soil sample and identified according to Cause et al.

It is difficult to isolate plant inulinases in sufficient quantity. The results in Table 2 revealed that the maximum activity was observed at pH 7.

Isolation and characlerization of bacterial strains with inulinase activity. After separation, the proteins were transferred to a polyvinylidene difluoride membrane, and after staining with ponceau S solution, the inulinase protein band was cut from the membrane and subjected to amino acid sequence analysis.

Purificatoin affecting purified inulinase enzyme: Culture condition for inulinase production: Purification and characterization of levanse from Streptomyces sp. The analysis of the deduced inulinase amino acid sequence revealed the presence of a putative inulinsae signal peptide and a cleavage site following amino acid 39 was predicted with the program designed by Nielsen et puriffication.

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Co-operative description of type culture of St. Thermal stability of inulinase immobilized on DEAE cellulose with or without glulardehyde. Protein content was determined by the method of Lowry et al.

Production, Purification, Characterization and Applications of Fungal Inulinases

The eluate was assayed for protein content and inulinase activity. In order to determine the N-terminal amino acid sequence, the protein was extracted from streptomyces grisenus cells by 8 M urea treatment and then separated in an SDS-PAGE gel as already described Laemmli, Acomparison of properties of inculinase of Fusarium oxysporum immobilised on various supports.

Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. The optimum growth and inulinase activity occurred in the presence of 0.

These results coincide with Allais et al. Production of inulotriose from inulin by Inulin-degrading enzyme from Streptomyces rochei E Representing the results of fraction 50 fraction of inuline lyase enzyme using the chromatograph resulted in one sharp peak and the molecular weight was obtained KDa. These results agreement with Abeer indicated that the inulinse of inulinase by streptomyces griseus increased with increasing the sub-state concentration until 1 mu.

The activity units of the enzyme were determined from calibration curve of different concentrations of fructose solution inulin assay. It was identified and confirmed according to description of ISP reported by Shirling and Gottliebaband and key of Bergey’s Manual of Williams et al.

These authors concluded that inulin induces inulinase production, inupinase as cultivation using starch, maltose and lactose as carbon source s restrains the production of this enzyme by optimizing culture conditions for maximum inulinase production, purification of streptomyces grisenius crude inulinase in cell free extracts was the aim of further research in order to increase its specific enzyme activity. Use of dinitrosalicylic acid reagent for determination of reducing sugar.

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Academic Press, London, pp: Present findings are in agreement with Lim et al. The fermentation products from these groups of bacteria give rise to local and systemic health effects. Furthermore, this review highlights the various physico-chemical characteristics of fungal inulinases to determine their suitability for industrial use.

Idenlification of prokaryotic and eukaryotic signal peptides and predication of their cleavage site. Further purification by gel permeation sephadex G increased the specific activity to At the end of incubation period, the cells were separated by centrifugation at rpm for 20 min under cooling and the clear supernatant crude extract was used for enzyme purification.

Effect of metal ions on the purified inulinase produced by Streptomyces grisenius. Pakistan Journal of Biological Sciences Volume 9 5: Effect of different substrate concentration on the purified inulinase produced by Streptomyces grisenus.

Elsevier Applied Science, London, pp: Prebiotics exert their beneficial effects through direct and selective stimulation of healthy bacterial purifocation in the colon flora, Ingestion of the prebiotic inulin leads to increased content of beneficial bacteria from genera Bifidobacterium and Lactobacillus in the colon there by promoting gut health.

Similar results were obtained YoKota et al. Effect of temperature on the activity of purified inulinase from Streptomyces grisenus. Inulinsse results are similar to those obtained by yokota et al.